Destiny Udogie is out of Saturday's Premier League clash against Wolves at Molineux (3pm UK).
The full-back picked up a muscular injury in last weekend's draw against Brighton.
It's the only additional injury concern for Roberto De Zerbi building up another crucial fixture.
Pape Matar Sarr remains out after missing the Brighton game with a shoulder issue. Guglielmo Vicario is closing in on a return to full training after a hernia repair.
Speaking at his pre-match press conference at Hotspur Way on Friday, Roberto said: "Destiny has a problem and can't play tomorrow. I'm sorry, because he played a great first half, second half as well, but the first one was great.
"We have Djed Spence, Souza is also available to come with us, so it's not a big problem. I hope Destiny will be available next week at Villa Park.
"It's a muscular injury, not so important. He didn't play for a long time and it can happen at the beginning when you feel something of this problem.
"I have a big opinion about Destiny - he's a crucial player for us. We are lucky because we can play Djed Spence, another important player who has played very well this season, another very good guy.
"Pape felt a pain in his shoulder an d didn't train with the group this week. He's not available."
Ben Davies, Dejan Kulusevski, Wilson Odobert, Mo Kudus and Cristian Romero remain sidelined.
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