Harry Kane suffered an ankle injury in a brief substitute appearance for England Under-21s against Wales on Monday night and is now unfortunately out of the Toulon Tournament.
The striker replaced Tom Ince in the 79th minute at Swansea's Liberty Stadium but picked up the injury - a sprained ankle - just minutes later after a challenge with Wales defender George Ray. He was replaced by Jake Foster-Caskey in the 84th minute.
England coach Gareth Southgate confirmed he would now miss out on the Toulon Tournament.
"It’s a real shame because Harry has been extremely patient for his chance and I was looking forward giving him some of the prestigious games in Toulon," Southgate told thefa.com.
"He’s warranted that opportunity and has had a super finish to the season with Tottenham so it’s disappointing for us and for him."
England beat Wales 3-1 thanks to a hat-trick from Norwich's Nathan Redmond to further strengthen their position at the top of Group One in qualifying for the 2015 European U21 Championships.