Benoit Assou-Ekotto and Sebastien Bassong suffered World Cup heartbreak on Saturday night.
The Spurs pair both started as Cameroon crashed out of the tournament with a 2-1 defeat against Denmark.
The result left Holland top of Group E on six points but means the Indomitable Lions cannot overhaul both Japan and the Danes, who will fight it out in the final group match for the right to join the Dutch in the last 16.
It could have been a different story. Samuel Eto'o opened the scoring for Cameroon in the 10th minute, Niclas Bendtner and Dennis Rommedahl turned it around for the Danes but Thomas Sorensen made two vital saves to keep the scoreline at 2-1 - an equaliser and Cameroon would still have had an outside chance to progress.
Instead, Benoit and Sebastien will be playing for pride in the final group game against Holland on Thursday.
The next Spurs interest in South Africa is with Heurelho Gomes and Brazil, although the goalkeeper is likely to be on the bench against Ivory Coast on Sunday evening.
Wilson Palacios and Honduras will realistically need to take something from Spain in their second match in Group H on Monday night.
The final round of group matches begin on Tuesday with Giovani dos Santos and Mexico up against Uruguay in Group A.