Dean Richards (eye), Robbie Keane (shoulder) and Jamie Redknapp (calf) are all struggling for the Turf Moor encounter after Sunday's Premiership clash against Chelsea.
Christian Ziege (calf), Ben Thatcher (back), Mauricio Taricco (groin) and Neil Sullivan (broken thumb) all remain out.
It appears unlikely that the likes of Steve Clemence, Darren Anderton and Ledley King, all back in action after injury, will be ready to step back into the first team fold.
Whoever plays on the night, boss Glenn insists the game and competition is 'as important as any Premiership match' to the club.
"This is an important cup for us," he stressed. "It's a route into Europe so we want to get it right at Burnley. We've a few injury concerns and at this time of year, perhaps it's time to utilise the squad.
"But I can assure you, whatever team we put out, we won't be taking this anywhere near a tad lightly. In fact this game is just as big a game as any in the Premiership for us because we've got to try to get into Europe this way as well."
Glenn Hoddle reports doubts over three players for Wednesday's tricky Worthington Cup trip to Burnley.