The midfielder missed Saturday's victory against Liverpool with a nasty gash on his shin and will not return for the season's finale at Filbert Street.
There was some positive injury news though as the boss reported that Ledley King (Achilles) and Dean Richards (knee, ankle) may be back in time.
Glenn said: "Ledley and Deano might have an outside chance but Tim is a definite no. He has a slight problem on the shin that is going into the muscle.
"There's not a lot coming out of the treatment room and for one game, I'm not sure we'll risk anyone.
"The boys who have performed in recent weeks have done magnificently because we've been down to our barest bones and they've responded."
Glenn Hoddle has ruled Tim Sherwood out of our final game at Leicester City on May 11.