The midfielder picked up a knee injury against Southampton and joins Ledley King (hamstring), Simon Davies (groin) and Kazu Toda (calf) on the sidelines.
Christian Ziege, who is back in match action after a second thigh operation, is not in the squad but has continued his progress in a private friendly at Leyton Orient this afternoon.
Caretaker boss David Pleat revealed this afternoon: "Jamie isn't in the squad. He got a kick on the knee and has a bit of inflammation and he won't travel.
"Gus Poyet is fit and returns and there is also a couple of youngsters in there in John Jackson and Jonathan Blondel."
Skipper Jamie Redknapp will miss Wednesday night's Carling Cup clash against Coventry City at Highfield Road.