Women's Super League side Reading's development team were the sixth and last opponents for Spurs ahead of Sunday's big kick-off in the FA Women's Premier League against Swindon Town.
Goals from in-form Kelley Blanchflower and the returning Wendy Martin saw Spurs home 2-0 at Frederick Knight Sports Ground.
Spurs have an unblemished record from pre-season encounters against Enfield, Middlesbrough and the development teams from WSL sides Arsenal, Chelsea, Watford and now Reading.
For Karen though, it's all about Swindon in their FAWPL opener at Brimsdown Rovers FC on Sunday (2pm).
"It's been a really positive pre-season but essentially it means nothing now and we have to produce the performance when it matters against Swindon on Sunday," she said.
"We had a sit down with the players after Sunday and reiterated that we've been delighted with their hard work in pre-season but now they have to take it out there when the league starts on Sunday.
"Swindon will be up for it and it's about putting in the level of performance we're looking for week in, week out."
Below: Lucia Leon celebrates with goalscorer Kelley Blanchflower
SPURS LADIES 2-0 READING DEVELOPMENT XI
Report from Khalid Karimullah, at Frederick Knights Sports Ground
All photos by and with thanks to Wu's Photography
Spurs Ladies ended their preparation ahead of the new season in fine fashion with a 2-0 win against WSL side Reading’s development squad.
Spurs started the brightest. Lucia Leon headed just wide from Eartha Pond's corner and Wendy Martin had a double chance a minute apart, first heading Pond’s corner over before glancing another Pond delivery just wide.
Martin then won but missed a penalty on the half-hour and Spurs went close again, this time Georgia Clifford made a double block to deny Renee Hector’s header from Pond’s corner, before clearing Whinnett’s follow-up.
The deserved goal followed on 40 minutes. Sophie McLean found Whinnett who skilfully flicked the ball into the path of the overlapping Shannon Moloney and her cross was tapped home by Kelley Blanchflower.
Just four minutes later, Leanne Mabey found Martin and her cross floated over Valentine and home for 2-0.
Daisy Mclachlan went close for Reading at the start of the second half but it was soon Spurs on the attack again. Pond’s free-kick fell to Chloe Gunn who fired over, Whinnett also couldn't keep a shot down and Blanchflower was denied a goal by the offside flag.
Toni-Anne Wayne did well to deny Clifford and Hannah Knapton rattled the crossbar late on.
Spurs Ladies: Toni-Anne Wayne, Shannon Moloney, Jenna Schillaci, Renne Hector, Ellie Butterwick, Sophie Mclean, Eartha Pond, Lucia Leon, Shakira Waithe, Wendy Martin, Nikita Whinnett, Chloe Gunn, Sophie Tudor, Emily Smith, Chelsea Grimes, Shona Chitate.