Anthony Gardner, Ben Thatcher, Alton Thelwell, Gary Doherty and Matthew Etherington are all set to feature as the team aim for a repeat of the 2-0 victory at Stevenage earlier in the season.
"We actually got a fairly experienced team to take there," reported Colin. "Jamie Slabber is expected to play along with one second year - other than that we've a side that everyone will know.
"I think we're going to have the same midfield as we've had in the last two games, which is pretty unusual. They've done particularly well.
"We've had a couple of good games. Wimbledon was a good performance and we went to Northampton and had a good competitive game against their first team.
"We played there last year and got beaten by one goal. The games against Southampton have been good and very nip and tuck. We did well to beat them when they had a strong side earlier this season.
"I know their coach Steve Wigley from my time at Nottingham Forest and, like anything when you know someone, he will want to beat me and I definitely want to beat him."
Spurs: Sullivan, Thelwell, Thatcher, Ricketts, Doherty, Gardner, Blondel, Sherwood, Slabber, Barnard, Etherington, Jackson, Burch, Henry, Hughes, Yeates.
Southampton: Jones, Dodd, Benali, Byles, Williams, Baird, Arias, Crowell, Delgado, K Davies, Howard. Subs: Mills, Blayney, Anderson, Gleeson, A Davies.
Colin Calderwood's reserve side will be seeking our first win at St Mary's when they head for the South Coast tonight to face Southampton, kick-off 7pm.