The amount was raised at two events held by the club at the end of this season.
At a recent Box Holder's event two bids of £10,000 and £15,000 were made to attend a training session with the first team squad next season, which helped double the club's pledge to the charity this year.
Along with funds raised through a match for Spurs fans at White Hart Lane the overall total was boosted to £50,000.
Skipper for 11 of his 16 memorable years at the Lane, Gary, who captained one of the fan's teams on the day of the match, handed over the cheque to Craig Paterson, Founder of Football Aid.
Craig commented: "This is an amazing amount of money and it will certainly help all the chosen charities nominated by both the charity and the Club"
A total of 31 Tottenham Hotspur supporters played on the hallowed turf of White Hart Lane back in April, making the most of a once in a lifetime opportunity.
The match resulted in a 7-1 victory for Gary's team, with the ex-England international scoring one of the goals against the team captained by fellow Lane legend Martin Chivers.
A social event held on April 25 for Box Holders and corporate guests was attended by many of the senior squad and legends from the past including Club President and double-winning manager Bill Nicholson.
Various auctions including the opportunity to train with the team and win a dream holiday supplied by new sponsors, Thomson, were held, resulting in £28,500 being raised for the charity.
The money generously pledged by Spurs fans will go to a mixture of diabetes charities, the FA's charity - The Princes Trust, Tottenham's local community voluntary organisations and the Club's own nominated beneficiaries.
Last year the charity raised a national total of £250,000, and allowed over 1200 people to wear their team colours, walk down the tunnel and play 90-minutes at their club ground. The expected national total this year is £500,000.
The legendary Gary Mabbutt made a return to White Hart Lane on Thursday to hand over a cheque for £49,900 to the Football Aid charity.