Goals from Kirsten Thomson and Emma Sherwood secured a 2-1 win against FA Premier League National Division side Watford in the League Cup.
It meant the ladies finished their League Cup campaign on a positive note after such a tough draw.
They were up against National Division teams Watford and Barnet plus Colchester and West Ham, who fought it out for the title in their own PL Southern Division.
"To win a game against a National Division team is such an achievement for us," said manager Karen Hills.
"It just shows how far we've come this season."
They can add silverware to what has been an outstanding campaign when they take on MSA Ladies in the final of the London Cup at Harrow Borough FC on Thursday week, March 29.
WOMEN'S FA PREMIER LEAGUE CUP
Watford 1-2 Spurs
Spurs Ladies came from 1-0 down to end their Premier League Cup campaign on a high against National Division Watford.
Once again, the ladies started well with Jess Cheeseman and Robyn McEvoy controlling the central midfield areas.
However, they conceded a sloppy goal from a set-piece to hand Watford the lead midway through the first half.
The team hit back with the move of the game to level just before half-time.
Vicky Elcome found Jess who switched play to Jenna Schillaci. She played the ball over the top for Kirsten Thomson who saw her initial effort saved by reaction well and headed home the rebound.
Emma Sherwood and Siobhan Wilson entered the fray for the second half and Emma made an instant impact - scoring what turned out to be the winner with her first touch.
As expected, Watford poured forward after that but Spurs stayed strong defensively to hold onto their lead and a deserved win.
Spurs Ladies: Jo Ayre, Ebony Heath, Vicky Elcome, Miranda Hall, Sam Pittuck, Jess Cheeseman, Robyn McEvoy, Derrie O'Hallaran (Nicola Cable, 75), Jenna Schillaci (Siobhan Wilson, 46), Wendy Martin, Kirsten Thomson (Emma Sherwood, 46).