Robbie Keane added to Berba's early strike as we took total control of the fixture.
However, home keeper Martin Vaniak produced a string of fine saves to keep the score at 2-0 and when Radek Cerny failed to deal with a high cross in thr 69th minute, David Strihavka tapped in for 2-1 to give Slavia hope on the night and they later struck the woodwork.
Despite that late rally, Jarolim accepted his side face a tough test. "I'm happy that we performed better in the second half but we've lost the first leg and the second leg will be very difficult," he said.
"We knew we were up against a very strong opponent and we planned to reinforce the midfield, not allow Tottenham to pass the ball around quickly and prevent them creating chances.
"Unfortunately we conceded that early goal and that knocked us back. Our plans were somewhat destroyed. The second goal put Tottenham in charge - the plan for midfield didn't work out and we allowed them too much time and space.
"We changed to a 4-4-2 for the second half, the players knew they had nothing to lose and we were more dangerous. If we had a little more luck we might have got a draw."
Slavia boss Karel Jarolim admitted his pre-match plans went out of the window when Dimitar Berbatov struck in the fourth minute of our UEFA Cup Round of 32 first leg in Prague.