The teams were nominated together for the award after their work saved the life of Fabrice Muamba, the Bolton midfielder who suffered a cardiac arrest on the pitch during our FA Cup quarter-final replay in March.
The awards took place in Manchester on Thursday night.
Wayne Diesel, Head of Medical Services, said: “What happened on that fateful night when Fabrice collapsed will live forever in our memories.
"More important than just a memory or receiving an award however is that we continue doing everything to promote awareness of how effective resuscitation with a 'simple' defibrillator can be in saving lives. Both clubs can be justifiably proud of the work carried out by their respective medical teams and support staff."
The UK Heart Safe Awards, supported by the defibrillator industry, are the first awards to celebrate organisations and employers who invest in creating Heart Safe environments as well as the acts of individuals who have saved lives while at work or leisure.
Dr Shabaaz Mughal, Club Doctor, Geoff Scott, Head Physio and members of the paramedic team at White Hart Lane will be presented with the award by David Howarth, chairman of Hand on Heart, during half-time in Sunday's match against QPR.
The work of Dr Shabaaz, Geoff and Wayne was also recognised at the recent BBC 999 Awards, again alongside Bolton's medical team.
To learn more about Hand On Heart, go to their website here...