Similar to last week's reverse against Fulham, Colin Calderwood's side produced most of the eye-catching football, but were unable to beat a familiar face in the Chelsea goal.
Neil Sullivan made his first start in Chelsea colours, while Darren Anderton and OJ Mabizela clocked up some playing minutes in an otherwise youthful second-string
Spurs were definitely the more fluent in the opening exchanges, the pick being a superb midfield combination on seven minutes between Johnnie Jackson and Anderton which presented Jamie Slabber with a shooting chance in front of goal, but his effort sailed into the arms of Sullivan.
The game then meandered slightly, coming to to life again on 20 minutes when Lee Barnard flashed a header wide following a good cross from Jackson. The latter then just failed to connect with an excellent right wing cross from Barnard.
Minutes later Barnard again acted as provider for Jackson, playing him in one on one with Sullivan, but the midfielder got too much height into his shot which clipped the top of the bar and went over.
It was Chelsea who took the lead - completely against the run of play - a minute before the break when an innocuous looking collision between Dean Marney and home left-back Sam Tillen was deemed by referee Mr Stroud to be a penalty offence - Sebastian Kneissl slotted home the spotkick to Rob Burch's right.
Mark Yeates was introduced after the break in the hope of adding some extra creative spark to the midfield and, as in the first period, all the slick passing and movement came from Spurs as the boys chased the elusive breakthrough.
Chelsea had a half chance to double their advantage when Joel Kitamirike broke into the right side of the area and Burch got down well to save. They did achieve this on 65 minutes when Filipe Oliveira delivered a pinpoint ball into the path of Kneissl who shot past Burch for his second of the game.
Team: Burch, Marney, McKie, Henry, O'Donoghue, Mabizela, Anderton, Hughes (Yeates 45), Jackson, Barnard, Slabber. Subs: Barnett, Eyre (GK), Hodges, Barcham.
Chelsea: Sullivan, Smith (Grant 52), Tillen, Kitamirike, Huth, Di Cesere, Oliveira, Gallaccio (McKinlay 52), Kneissl (Hollands 78), Keenan (C), Rocastle. Subs: Watt (GK).
For the second week running the reserves failed to gather any rewards for their endeavours - falling to an ill-deserved defeat to Chelsea at Aldershot on Monday evening.