Les suffered a nasty blow to the knee after a challenge from Villa’s Alan Wright in Saturday’s Premiership opener.
The striker and skipper on the day was determined to shake the problem off but was clearly struggling and substituted after 20 minutes.
Boss Glenn revealed: “Les took a nasty blow just above the knee but there’s nothing twisted, nothing of too much damage.
“What I will say, with us playing Monday, he has to be very, very doubtful. That’s gone against us, which is a blow.”
Glenn was more positive about Teddy, who missed Saturday’s goalless draw after pre-season Achilles problems and Darren Anderton, who suffered similar problems but was able to play the final 20 minutes against Villa.
“Teddy is hopeful,” said Glenn. “Darren was a little bonus but he hasn’t had much football to play at that tempo.
“We’ve got to make sure we look at who is fit, there are a few knocks and bruises in there and unfortunately when you are playing 48 hours later, there is not much time to recover so it will be difficult.”
Glenn Hoddle has already labelled Les Ferdinand’s chances of playing at Everton as ‘very, very doubtful’ but has better news about Teddy Sheringham.