But our outstanding midfielder/defender assured the Official Spurs Website that it was only a dead leg and that he expects to travel to Albania with the rest of the squad for Tuesday's qualifying fixture.
"It's nothing too much to worry about, just a dead leg," he revealed. "I'm expecting to travel to Albania."
Starting at centre-back in a back four alongside Spurs team-mate Luke Young, Ledley was untroubled before his early departure.
Luke, meanwhile, pushed further and further on in his right-back role as the game progressed.
England eventually won 4-0 after dominating from start to finish. Darius Vassell opened the scoring in the 27th minute, skipper John Terry made it 2-0 after 74 and Shola Ameobi grabbed a late double after coming off the bench to seal a good night's work.
The result lifted England up to third in Group Nine. Germany top the table after hammering Albania 8-0.
Injury forced Ledley King off at half-time in England Under-21's excellent win against Finland at Barnsley on Friday night.