Both were named by boss David Platt in a 24-man squad on Friday for European Championship qualifiers away to Germany on Friday and against Albania at Middlesbrough on Tuesday week (September 4).
Ledley, outstanding so far this term, will aim for his eighth cap after missing out on the last squad because of injury.
Alton, yet to feature in the Premiership this season, is recalled to the squad after winning his only cap against Spain last season.
Squad: Gareth Barry (Aston Villa), Titus Bramble (Ipswich Town), Wayne Bridge (Southampton), Stephen Bywater (West Ham), Luke Chadwick (Manchester United), Malcolm Christie (Derby County), Joe Cole (West Ham), Sean Davis (Fulham), Jermain Defoe (West Ham), Jonathan Greening (Middlesbrough), Andy Griffin (Newcastle), Francis Jeffers (Arsenal), Seth Johnson (Derby County), Ledley King (Tottrenham Hotspur), Scott Parker (Charlton Athletic), Jermaine Pennant (Arsenal), David Prutton (Nottingham Forest), Chris Riggott (Derby County), Stuart Taylor (Arsenal), Alton Thelwell (Tottenham Hotspur), Darius Vassell (Aston Villa), Nick Weaver (Manchester City), Mark Wilson (Middlesbrough), Stephen Wright (Liverpool).
Ledley King and Alton Thelwell are back in the England Under-21 squad.