It's the first time the team have progressed this far in the ladies' showpiece competition since reaching the fifth round in successive years in the mid-1990s where their progress was twice halted by Croydon Ladies, the big guns of the women's game at the time.
The class of 2015 are enjoying one of the best seasons in the history of the club as they remain in the shake-up for a top four finish in the FA Women's Premier League South and have reached the final of the Ryman Senior Women's Cup, where they take on Charlton on April 16.
For now, full focus is on the FA Cup and a trip to a Derby team currently 10th of 12 in the FAWPL North but no doubt buoyed by last week's 3-0 win against Wolves.
Meanwhile, for Spurs, it's been a tough couple of weeks on and off the pitch since their last fixture - the Ryman Cup semi-final victory against Gillingham on February 12.
That win made it four on the trot but they haven't played since then after a succession of postponed matches while off the pitch, the club as a whole was hit by the sudden, sad passing of chairman Glenn Weaver.
"It's been a difficult time for everyone and we're now all looking forward to getting back into action," said manager, Karen Hills, who described Glenn as 'irreplaceable'.
"There is now huge excitement about this FA Cup tie. I don't recall playing Derby before and this is what the FA Cup is all about.
"Derby haven't had the best of runs but they picked up a good result against Wolves last week. I know they've also had a couple of run-outs against Women's Super League teams, so they will be prepared and ready for us.
"We'll just concentrate on what we need to do. We're in form and we have players who can make a difference. We know if we play to our capability we can give any team a game.
"We've been without a game for a couple of weeks but we're in form, we've trained well and the girls can't wait for this one."
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