Paul Robinson saved a penalty as England fell to a single goal defeat to Spain in the friendly international in Madrid.
Del Horno scored the decisive goal in the 10th minute but events on the pitch at the Bernabeu Stadium were overshadowed by racist taunts aimed in the direction of England's black players.
The Spain left back headed in his goal after the England rearguard failed to deal with a cross from the left that bounced in the area and his effort lofted over the despairing dive of Robinson.
On 25 minutes our goalkeeper advanced from his line to deny Raul a shot on goal but the referee deemed this illegal and awarded a penalty. Raul stepped up, fired to his right and Robinson sprang across his goal to make the save.
The home side pretty much dominated the game and England struggled to make in-roads despite the introduction of Jermain Defoe with 12 minutes remaining.
Kasey Keller was on duty for the USA as they progressed to the final phase of qualification in the North, Central American and Caribbean Zone with a 1-1 draw with Jamaica, who are now eliminated from the World Cup.
Thimothee Atouba's Cameroon were defeated 3-0 in their friendly international with Germany in Leipzig and manager Winfried Schaefer was sacked after the game.