The in-form front man, EA SPORTS Player of the Month in the Premier League for February, limped out of last week's FA Cup clash with Millwall and is sidelined for the visit of the Saints.
Nevertheless Puel, who saw his side beaten 4-1 by us at St Mary's just after Christmas, is expecting a difficult test.
Asked about Harry's absence ahead of the match, he said: “He’s an important player for them, but I think they have other solutions.
“In the last game you could see Son with three goals and with Alli and Janssen also, Eriksen too, they have many quality offensive players and they can do without him.
“He’s a strong and great player of course, but there are many solutions for Pochettino.”
Southampton were the last team to beat us at White Hart Lane back in May and, speaking about Sunday’s contest in general, former Lyon and Nice chief Puel added: “It’s a good game, a good challenge for us.
“It’s a difficult game but we like this, with the possibility to see if we have made progress since the beginning of the season, since the winter, when we lost at home against them. They were superior and now it’s interesting to see our progress.”