The midfielder - only just back in the England squad after missing the summer's European Championships to rest an Achilles problem - had to be substituted just 12 minutes into the thrilling 3-2 victory at White Hart Lane.
Physio Alasdair Beattie confirmed that Anderton would undergo an MRI scan on Wednesday morning to reveal the full extent of the injury.
It seemed initially that Anderton was injured after being clattered by Everton defender David Unsworth in the fifth minute but manager George Graham insisted the problem arose even earlier than that.
Graham, clearly fearing the worst, admitted: "Darren has picked up what we think is a bad groin injury. It wasn't in the collision with Unsworth though - it was in the first minute taking a corner."
The news on Ben Thatcher was slightly more encouraging. The left-back also had to be substituted in the first half suffering from chest pains after a clash with Steve Watson. Physio Beattie revealed that Thatcher was struggling to breathe but expected him to be available for selection in time for Monday's visit of West Ham. Boss Graham added: "Ben wanted to carry on but couldn't."
ASK LUKE: Our Mr Versatile and now international goalscorer is in the hotseat to take your questions this weekend.
Email your questions to Luke here.
There are fears at the Lane that Darren Anderton may be out of action for some time after picking up a groin injury against Everton on Tuesday night.