The lads found themselves 2-0 down against a Bees side that included a number of first team players before Andy Barcham sparked a revival. However, despite pushing forward in the latter stages, an equaliser proved elusive with Jamie O'Hara and Stuart Lewis both going close.
Development coach Clive reflected: "You knew their lads wanted to make an impression, they had a lot of first team players and wanted to do things in the right way.
"That's exactly what we wanted and it was a good game for us in a free week before a couple of important reserve matches coming up against Fulham and Chelsea.
"There are lessons to be learned and when you are defeated it highlights things, the lads are disappointed and know they didn't play as well as they can.
"We will talk about it, work on things and losing is all part of the game - you can't win every time and I'd rather lose in a training ground friendly than a league game, that's for sure."
Spurs: Burch, Ifil, Ziegler, Davenport (Mills, 45), Dervite, McKenna, Murphy (Pekhart, 60), O'Hara, Lewis, Daniels, Barcham.
Brentford: Masters, Tillen, O'Connor, Hayward, Frampton, Rhodes, Moussinho, Cox, Brooker, Moore, Wyjnard.
Clive Allen said he learned a lot from a rare defeat for his reserve side in a training ground friendly against Brentford on Tuesday.